Detection of subpicogram quantities of specific DNA sequences on blot hybridization with biotinylated probes

Detection of subpicogram quantities of specific DNA sequences on blot hybridization with biotinylated probes

A sensitive method for detecting biotinylated DNA probes on dot and Southern blots is described. This method is fast, reproducible and can detect single copy genes in 25 mu g of human genomic DNA on Southern blots.

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Veröffentlicht in:Nucleic acids research 1985-01, Vol.13 (22), p.8083-8092
Hauptverfasser: Chan, VI-W, Fleming, KA, McGee, JO'D
Format: Artikel
Sprache:eng
Schlagworte:
DNA
probes
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Beschreibung
Zusammenfassung:A sensitive method for detecting biotinylated DNA probes on dot and Southern blots is described. This method is fast, reproducible and can detect single copy genes in 25 mu g of human genomic DNA on Southern blots.
ISSN:0305-1048
DOI:10.1093/nar/13.22.8083