A Synthetic Peptide Vaccine Involving the Product of the Pre-S(2) Region of Hepatitis B Virus DNA: Protective Efficacy in Chimpanzees

The S gene encoding the major surface polypeptide of hepatitis B virus is preceded by the region pre-S(2) with a capacity to code for 55 amino acid residues. In the product of region pre-S(2), the sequence of 19 amino acid residues (amino acids 14-32 from the N terminus) representing an area of high...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1986-12, Vol.83 (23), p.9174-9178
Hauptverfasser: Itoh, Yukio, Takai, Emiko, Ohnuma, Hitoshi, Kitajima, Kohji, Tsuda, Fumio, Machida, Atsuhiko, Mishiro, Shunji, Nakamura, Tetsuo, Miyakawa, Yuzo, Mayumi, Makoto
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Sprache:eng
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Zusammenfassung:The S gene encoding the major surface polypeptide of hepatitis B virus is preceded by the region pre-S(2) with a capacity to code for 55 amino acid residues. In the product of region pre-S(2), the sequence of 19 amino acid residues (amino acids 14-32 from the N terminus) representing an area of high local hydrophilicity is shared by viral strains of subtypes adr, ayw, and ayr; residue 22, phenylalanine, is replaced by leucine in a strain of the other subtype, adw. A synthetic peptide vaccine involving these 19 amino acid residues, when given to two chimpanzees, raised antibodies that bound to viral particles and protected the animals from challenge with 106 chimpanzee infectious doses of hepatitis B virus.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.23.9174