Immunocytochemical localization of Ras super(Ha) p21 in normal and neoplastic cells in fixed tissue sections from Harvey sarcoma virus infected mice

An affinity purified sheep IgG antibody to a 20 amino acid peptide from the carboxyterminal end of Ras super(Ha) p21 was used to localize Ras super(Ha) p21 on fixed tissue sections of Harvey sarcoma (HaSV) virus-infected mice by the avidin-biotin-peroxidase immunocytochemical technique. Control sera included immune sheep sera absorbed with the peptide, preimmune sheep area and a goat polyclonal antibody to Rauscher leukemia virus p30. Neonatal BALB/c mice were injected with HaSV/Moloney leukemia virus (MoLV), MoLV alone or buffer. With the use of affinity purified IgG sheep polyclonal antibody to a peptide in Ras super(Ha) the authors were able to demonstrate Ras super(Ha) p21 in tumors and other cells. The degree of immunoreactivity was related to the expected level of p21 expression.