Plasmid stability of Bacillus thuringiensis var. kurstaki (HD-1) during continuous phased cultivation

Bacillus thuringiensis var. kurstaki (HD-1) was grown as a continuous phased culture in a cyclone fermentor. During the time course of the continuous phased cultivation (CPC), the culture was sampled to determine the efficiency of sporulation and parasporal crystal formation. The data suggests that...

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Veröffentlicht in:	Biotechnology letters 1987-01, Vol.9 (7), p.483-488
Hauptverfasser:	Roy, B.P, Selinger, L.B, Khachatourians, G.G
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus thuringiensis Bacillus thuringiensis kurstaki Biological and medical sciences Biotechnology cultivation culture media Fundamental and applied biological sciences. Psychology genetic code Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology plasmids sporulation stability
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Zusammenfassung:	Bacillus thuringiensis var. kurstaki (HD-1) was grown as a continuous phased culture in a cyclone fermentor. During the time course of the continuous phased cultivation (CPC), the culture was sampled to determine the efficiency of sporulation and parasporal crystal formation. The data suggests that the disappearance of parasporal crystals in B. thuringiensis var. kurstaki (HD-1) during CPC occurs independent of plasmid copy but may be due to defective sporulation.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF01027457