The investigation molecular epidemiology of ESBLs in Chizhou 2010-2012

Objective To study the molecular epidemiology of ESBLs about Escherichia coli and Klebsiella pneumoniac in Chizhou. Methods 294 Escherichia coli strains and 178 Klebsiella pneumoniac strains were detected by ATB Expression bacterium analytical system from 2010 to 2012. Method of National Committee for Clinical Laboratory Standards was performed for screening and ascertainment of Escherichia coli and Klebsiella pneumoniac with ESBLs. Plasmid of the bacterium with ESBLs was extracted and amplified by polymerase chain reaction. Nucleate electrophoretic analysis was performed for genotyping of ESBLs. Results The percent of ESBLs positive Escherichia coli was 17.3%, of Klebsiella pneumoniac was 19.7%. Among the Escherichia coli strains, TEM type was 49.0%, SHV type was 37.2%, CTX-M type was 39.2%. Among the Klebsiella pneumoniac strains, TEM type was 48.6%, SHV type was 25.7%, CTX-M type was 42.8%. Conclusion The local ESBLs producing strains could have reached a certain proportion. Some strains might carry two or