The detection of the levels of plasma Ang II and AT1R mRNA expression after the compression of rabbit's vagus nerve

Objective To establish an animal model by fixing vagus nerve with connective tissue to make an cross-compression of vagus nerve and arteria carotis, and then to detect the levels of Ang II and the mRNA expression of ATIR. Methods As the surgery group, 25 New Zealand rabbits' vagus nerves were fixed with connective tissue to make persistent compression of vagus nerve by arteria carotis. Radioimmunoassay was applied to detect Ang II level and RT-PCR to detect the expression of AT1R mRNA. Mean-while 25 New Zealand rabbits without surgical treatment were used as controls. Results The level of plasma Ang II and myocardial AT1R mRNA expression were enhanced significantly compared with controls. Conclusion The persistent compression from arteria carotis to the demyelinated vagus nerve of left cervical part could enhance the levels of Ang II and mRNA of AT1R.