Analysis of Picogram Quantities of Indole-3-acetic Acid by High Performance Liquid Chromatography-Fluorescence Procedures

Analysis of Picogram Quantities of Indole-3-acetic Acid by High Performance Liquid Chromatography-Fluorescence Procedures

The use of spectrofluorimeter coupled to a reverse phase high performance liquid chromatography column permits selective detection of indole-3-acetic acid at the low picogram level. The value of the technique is demonstrated by the analysis of endogenous IAA in elongating shoots, xylem sap and callu...

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Veröffentlicht in:Planta 1980-12, Vol.150 (5), p.366-370
Hauptverfasser: Crozier, Alan, Loferski, Karen, Zaerr, Joe B., Morris, Roy O.
Format: Artikel
Sprache:eng
Schlagworte:
Acetates
Callus
Esters
Ethanol
Flow velocity
Fluorescence
Insulin antibodies
Mass spectra
Plants
Quaternary ammonium compounds
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Zusammenfassung:The use of spectrofluorimeter coupled to a reverse phase high performance liquid chromatography column permits selective detection of indole-3-acetic acid at the low picogram level. The value of the technique is demonstrated by the analysis of endogenous IAA in elongating shoots, xylem sap and callus of Douglas-fir. The data are also used to illustrate a procedure whereby the accuracy of chromatographic analyses can be verified within definable probability limits.
ISSN:0032-0935
1432-2048
DOI:10.1007/bf00390171