Energy barriers and driving forces in tRNA translocation through the ribosome
Using 13 intermediate-translocation-state models derived from X-ray and cryo-EM structures of Escherichia coli ribosomes to guide large-scale molecular dynamics simulations, a new study now models the path taken by tRNAs during spontaneous translocation to uncover the mechanisms that facilitate tRNA...
Gespeichert in:
Veröffentlicht in: | Nature structural & molecular biology 2013-12, Vol.20 (12), p.1390-1396 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Using 13 intermediate-translocation-state models derived from X-ray and cryo-EM structures of
Escherichia coli
ribosomes to guide large-scale molecular dynamics simulations, a new study now models the path taken by tRNAs during spontaneous translocation to uncover the mechanisms that facilitate tRNA movement through the ribosome.
During protein synthesis, tRNAs move from the ribosome's aminoacyl to peptidyl to exit sites. Here we investigate conformational motions during spontaneous translocation, using molecular dynamics simulations of 13 intermediate-translocation-state models obtained by combining
Escherichia coli
ribosome crystal structures with cryo-EM data. Resolving fast transitions between states, we find that tRNA motions govern the transition rates within the pre- and post-translocation states. Intersubunit rotations and L1-stalk motion exhibit fast intrinsic submicrosecond dynamics. The L1 stalk drives the tRNA from the peptidyl site and links intersubunit rotation to translocation. Displacement of tRNAs is controlled by 'sliding' and 'stepping' mechanisms involving conserved L16, L5 and L1 residues, thus ensuring binding to the ribosome despite large-scale tRNA movement. Our results complement structural data with a time axis, intrinsic transition rates and molecular forces, revealing correlated functional motions inaccessible by other means. |
---|---|
ISSN: | 1545-9993 1545-9985 |
DOI: | 10.1038/nsmb.2690 |