Anatomical modifications, viviparous reproduction and hydraulic expulsion of larvae by Cephenemyia nasopharyngeal bot flies of deer

Several specialized adaptations of the reproductive and respiratory systems associated with the retention and expulsion of larvae in ovoviviparous Cephenemyia species (Diptera: Oestridae) are described and illustrated. In these flies the anterior section of the common oviduct is modified into a large sac‐like uterus that contains larvae, and the posterior section is modified into a larvipositor with a central tubular vagina. During larviposition, contraction of abdominal muscles forces haemolymph into a perivaginal sinus, causing a hydraulically driven exsertion of the larvipositor. A group of larvae and uterine fluid sealed off within the lumen of the vagina are then expelled from the vulva via hydraulic pressure as the stretched vagina is compressed. A one‐way, non‐return valve between the uterus and vagina prevents a reflux of larvae upward into the uterus during larviposition. All mutually dependent actions associated with larviposition occur almost simultaneously. All species have evolved a similar mechanism of expelling their larvae, but the shape of the non‐return valve is different in each species studied.