Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy

Raman microscopy was used as a label-free method to study the mineralisation of bone nodules formed by mesenchymal stem cells cultured in osteogenic medium in vitro. Monitoring individual bone nodules over 28 days revealed temporal and spatial changes in the crystalline phase of the hydroxyapatite c...

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Veröffentlicht in:	Analyst (London) 2014-01, Vol.139 (1), p.55-58
Hauptverfasser:	Ghita, Adrian, Pascut, Flavius C, Sottile, Virginie, Notingher, Ioan
Format:	Artikel
Sprache:	eng
Schlagworte:	Bone Density - physiology Cell Size Cells, Cultured Humans Mesenchymal Stem Cells - chemistry Mesenchymal Stem Cells - physiology Spectrum Analysis, Raman - methods Time Factors
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Raman microscopy was used as a label-free method to study the mineralisation of bone nodules formed by mesenchymal stem cells cultured in osteogenic medium in vitro. Monitoring individual bone nodules over 28 days revealed temporal and spatial changes in the crystalline phase of the hydroxyapatite components of the nodules.
ISSN:	0003-2654 1364-5528
DOI:	10.1039/c3an01716h