Use of isopropyl-N(3-chlorophenyl) carbamate (CIPC) to produce partial haploid cells from suspension cultures of soybean (Glycine max)

Suspension cultures of a heterozygous line of soybean (Glycine max) were treated with CIPC. Surviving clones had lost 10-15 chromosomes. A recessive phenotype (non-fluorescence) not expressed in the heterozygote, was expressed with high frequency (15-20%) in these clones. Nucleoside auxotrophs were isolated from the segregant clones consistent with partial haploidy. A selective screen for nucleoside auxotrophs is described.