Production of extracellular fibers by tobacco leaf epidermal protoplasts

Production of extracellular fibers by tobacco leaf epidermal protoplasts

Protoplasts prepared from the upper epidermis of tobacco (Nicotiana tabacum L.) leaves produce tubular structures which are highly fluorescent in the presence of Calcofluor white. The presence of Ca2+ in the incubation medium appears to be necessary for the production of these fibers. A commercial c...

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Veröffentlicht in:Planta 1983-11, Vol.159 (3), p.282-285
Hauptverfasser: Fannin, F.F. (Kentucky Univ., Lexington (USA). Dept. of Plant Pathology), Shaw, J.G
Format: Artikel
Sprache:eng
Schlagworte:
BLATT
Cell walls
EPIDERMIS
INHALTSSTOFFSYNTHESE
Materials
NICOTIANA
Plants
PROTOPLAST
Protoplasts
Short communication
Soybeans
Tubular structures
ZELLE
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Zusammenfassung:Protoplasts prepared from the upper epidermis of tobacco (Nicotiana tabacum L.) leaves produce tubular structures which are highly fluorescent in the presence of Calcofluor white. The presence of Ca2+ in the incubation medium appears to be necessary for the production of these fibers. A commercial cellulase preparation destroyed these structures and inhibited their production.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00397537