Flow-cytometric characterization and sorting of plant chromosomes
Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytome...
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| Veröffentlicht in: | Theoretical and applied genetics 1984-03, Vol.67 (5), p.463-467 |
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| container_title | Theoretical and applied genetics |
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| creator | Laat, A.M.M. de Blaas, J |
| description | Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed. |
| doi_str_mv | 10.1007/BF00263414 |
| format | Article |
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Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>chromosomes</subject><subject>Cytogenetics</subject><subject>flow cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genetics and breeding of economic plants</subject><subject>Genetics of eukaryotes. 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Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>chromosomes</topic><topic>Cytogenetics</topic><topic>flow cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genetics and breeding of economic plants</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Haplopappus gracilis</topic><topic>plant breeding</topic><topic>Vegetals and fungi</topic><topic>Xanthisma gracile</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laat, A.M.M. de</creatorcontrib><creatorcontrib>Blaas, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laat, A.M.M. de</au><au>Blaas, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow-cytometric characterization and sorting of plant chromosomes</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>1984-03</date><risdate>1984</risdate><volume>67</volume><issue>5</issue><spage>463</spage><epage>467</epage><pages>463-467</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>24258767</pmid><doi>10.1007/BF00263414</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Theoretical and applied genetics, 1984-03, Vol.67 (5), p.463-467 |
| issn | 0040-5752 1432-2242 |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences chromosomes Cytogenetics flow cytometry Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetics and breeding of economic plants Genetics of eukaryotes. Biological and molecular evolution Haplopappus gracilis plant breeding Vegetals and fungi Xanthisma gracile |
| title | Flow-cytometric characterization and sorting of plant chromosomes |
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