Flow-cytometric characterization and sorting of plant chromosomes

Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytome...

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Veröffentlicht in:	Theoretical and applied genetics 1984-03, Vol.67 (5), p.463-467
Hauptverfasser:	Laat, A.M.M. de, Blaas, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences chromosomes Cytogenetics flow cytometry Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetics and breeding of economic plants Genetics of eukaryotes. Biological and molecular evolution Haplopappus gracilis plant breeding Vegetals and fungi Xanthisma gracile
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Beschreibung
Zusammenfassung:	Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.
ISSN:	0040-5752 1432-2242
DOI:	10.1007/BF00263414