Electrochemical real-time nucleic acid amplification: towards point-of-care quantification of pathogens

Highlights • We review current methods for electrochemical real-time nucleic acid amplification. • Electrochemical detection rivals optical methods due to low cost and complexity. • Initial methods were solid phase and suffered from low amplification efficiency. • New solution phase methods achieve...

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Veröffentlicht in:	Trends in biotechnology (Regular ed.) 2013-12, Vol.31 (12), p.704-712
Hauptverfasser:	Patterson, Adriana S, Hsieh, Kuangwen, Soh, H. Tom, Plaxco, Kevin W
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Catalytic oxidation Cytomegalovirus Deoxyribonucleic acid DNA Electrochemistry Electrodes Fundamental and applied biological sciences. Psychology gene amplification Hybridization Internal Medicine loop-mediated isothermal amplification Methods monitoring Nucleic Acid Amplification Techniques Nucleic acids pathogen detection Pathogens point-of-care Point-of-Care Systems quantitative polymerase chain reaction Real time real-time amplification Real-Time Polymerase Chain Reaction RNA
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Beschreibung
Zusammenfassung:	Highlights • We review current methods for electrochemical real-time nucleic acid amplification. • Electrochemical detection rivals optical methods due to low cost and complexity. • Initial methods were solid phase and suffered from low amplification efficiency. • New solution phase methods achieve clinically relevant sensitivity and specificity. • Solution phase methods can pave the road towards use in point-of-care applications.
ISSN:	0167-7799 1879-3096
DOI:	10.1016/j.tibtech.2013.09.005