Mapping of an ultrasonic bath for ultrasound assisted extraction of mangiferin from Mangifera indica leaves

•Mapping of ultrasonic bath for ultrasound assisted extraction of natural product.•Optimization of parameters for maximum extraction.•Position and shape of vessel has significant impact on extraction yield.•Mapping of ultrasonic bath can help to obtain maximum extraction yield. The present work deals with the mapping of an ultrasonic bath for the maximum extraction of mangiferin from Mangifera indica leaves. I3− liberation experiments (chemical transformations) and extraction (physical transformations) were carried out at different locations in an ultrasonic bath and compared. The experimental findings indicated a similar trend in variation in an ultrasonic bath by both these methods. Various parameters such as position and depth of vessel in an ultrasonic bath, diameter and shape of a vessel, frequency and input power which affect the extraction yield have been studied in detail. Maximum yield of mangiferin obtained was approximately 31mg/g at optimized parameters: distance of 2.54cm above the bottom of the bath, 7cm diameter of vessel, flat bottom vessel, 6.35cm liquid height, 122W input power and 25kHz frequency. The present work indicates that the position and depth of vessel in an ultrasonic bath, diameter and shape of a vessel, frequency and input power have significant effect on the extraction yield. This work can be used as a base for all ultrasonic baths to obtain maximum efficiency for ultrasound assisted extraction.