Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids within Brassicaceae

Fourty-one somatic hybrids from two species combinations, Brassica oleracea + B. campestris and B. napus + Eruca sativa, were analysed for chromosome number and nuclear DNA content. The DNA content was measured in a flow cytometer using two internal standards as references and when related to the ch...

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Veröffentlicht in:	Plant cell reports 1988, Vol.7 (1), p.74-77
Hauptverfasser:	Fahleson, J, Dixelius, J, Sundberg, E, Glimelius, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology Brassica napus Brassica oleracea Brassica rapa subsp. campestris chromosome number Classical genetics, quantitative genetics, hybrids DNA Eruca vesicaria Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Genetics of eukaryotes. Biological and molecular evolution Haploidy, in vitro culture applications, somatic hybrids Methods. Procedures. Technologies Miscellaneous nucleic acids Plant breeding: fundamental aspects and methodology Plant cells and fungal cells Pteridophyta, spermatophyta somatic hybridization Vegetals
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Zusammenfassung:	Fourty-one somatic hybrids from two species combinations, Brassica oleracea + B. campestris and B. napus + Eruca sativa, were analysed for chromosome number and nuclear DNA content. The DNA content was measured in a flow cytometer using two internal standards as references and when related to the chromosome number a correlation of 0.91 was found. The chromosome number of the hybrids could be determined with an accuracy of ±10% by using flow cytometry, and the smallest statistically significant difference in DNA content between two individuals was 0.23 pg DNA/cell.
ISSN:	0721-7714 1432-203X
DOI:	10.1007/bf00272983