Prophase bands of microtubules occur in protoplast cultures of Vicia hajastana Grossh

Circumnuclear bands of microtubules (MT) have been found in the prophase of mitoses in cultured protoplasts of Vicia hajastana. The timing of the appearance and disappearance of the prophase band of MT (PB) relative to the stage of mitosis was studied using simultaneous staining of MT by immunofluor...

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Veröffentlicht in:	Planta 1986-04, Vol.167 (4), p.469-472
1. Verfasser:	Simmonds, D.H. (Carleton Univ., Ottawa (Canada). Biology Dept.)
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences CELL DIVISION Cell kinetics Cell physiology CELL STRUCTURE Chromatin CULTIVO DE TEJIDOS CULTURE DE TISSUS Cultured cells DIVISION CELLULAIRE DIVISION CELULAR ESTRUCTURA CELULAR Fluorescence Fundamental and applied biological sciences. Psychology Guard cells Microtubules Mikrotubuli Mother cells Plant cells Plant physiology and development Prophase PROTOPLASM PROTOPLASMA PROTOPLASME Protoplast Protoplast culture Protoplasts Stoerung STRUCTURE CELLULAIRE Suspensionskultur Teilungsstadium TISSUE CULTURE VICIA Zellmerkmal Zellteilung
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Zusammenfassung:	Circumnuclear bands of microtubules (MT) have been found in the prophase of mitoses in cultured protoplasts of Vicia hajastana. The timing of the appearance and disappearance of the prophase band of MT (PB) relative to the stage of mitosis was studied using simultaneous staining of MT by immunofluorescence and DNA by Hoechst 33258. These protoplasts regenerate into unorganized tissue. Pre-prophase bands of MT have previously been found only in highly organized tissues of higher plants. The role of PB in cell division is discussed.
ISSN:	0032-0935 1432-2048
DOI:	10.1007/BF00391222