Measurement of the binding of DNA to liposomes by resonance energy transfer

Measurement of the binding of DNA to liposomes by resonance energy transfer

A rapid and simple semiquantitative method for monitoring the attachment of DNA to liposomes is described. This technique is based on the measurement of resonance energy transfer between a membrane-embedded fluorescent pyrene lipid donor and DNA labeled with adriamycin as an acceptor.

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Veröffentlicht in:Journal of fluorescence 1994-12, Vol.4 (4), p.345-347
Hauptverfasser: Köiv, A, Kinnunen, P K
Format: Artikel
Sprache:eng
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Zusammenfassung:A rapid and simple semiquantitative method for monitoring the attachment of DNA to liposomes is described. This technique is based on the measurement of resonance energy transfer between a membrane-embedded fluorescent pyrene lipid donor and DNA labeled with adriamycin as an acceptor.
ISSN:1053-0509
1573-4994
DOI:10.1007/BF01881453