Restriction endonuclease analysis of mitochondrial DNA from sorghum with fertile and male-sterile cytoplasms

Mitochondrial DNA from four paired (fertile and male-sterile) lines and six isocytoplasmic strains of sorghum (Sorghum bicolor (L.) Moench) were fragmented by endonucleases and their electrophoretic patterns were examined. Cytoplasmic male sterile lines differed from their male-fertile counterparts...

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Veröffentlicht in:	Theoretical and applied genetics 1989-03, Vol.77 (3), p.379-382
Hauptverfasser:	Lee, S.H, Muthukrishnan, S, Sorensen, E.L, Liang, G.H
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences chemical analysis Classical genetics, quantitative genetics, hybrids cultivars cytoplasmic male sterility DNA endonuclease Fundamental and applied biological sciences. Psychology genetic techniques and protocols Genetics and breeding of economic plants Genetics of eukaryotes. Biological and molecular evolution Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia mitochondria Plant breeding: fundamental aspects and methodology plant fertility Pteridophyta, spermatophyta Sorghum bicolor Vegetals
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Zusammenfassung:	Mitochondrial DNA from four paired (fertile and male-sterile) lines and six isocytoplasmic strains of sorghum (Sorghum bicolor (L.) Moench) were fragmented by endonucleases and their electrophoretic patterns were examined. Cytoplasmic male sterile lines differed from their male-fertile counterparts consistently. Among the isocytoplasmic strains, KS 36A (S. verticilli-florum cytoplasm), KS 38A (S. conspicum cytoplasm), and KS 39A (S. niloticum cytoplasm) showed minor differences from the other strains. Results suggest that restriction endonuclease patterns are useful in detecting differences in mitochondrial genomes.
ISSN:	0040-5752 1432-2242
DOI:	10.1007/BF00305832