Optimum tissue culture conditions for selection of resistance to Phytophtora cinnamomi in pine callus tissue

The present experimentation compared the best nutrient medium, temperature, and growth hormones for callus induction and growth of various pine species from different seed sources with their effect on growth of Phytophthora cinnamomi. Callus tissues maintained on a modified Murashige and Skoog mediu...

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Veröffentlicht in:	Plant cell reports 1991, Vol.9 (9), p.488-491
Hauptverfasser:	JANG, J. C, TAINTER, F. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Establishment of new cell lines, improvement of cultural methods, mass culture Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Plant cells and fungal cells
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Beschreibung
Zusammenfassung:	The present experimentation compared the best nutrient medium, temperature, and growth hormones for callus induction and growth of various pine species from different seed sources with their effect on growth of Phytophthora cinnamomi. Callus tissues maintained on a modified Murashige and Skoog medium with 10(-5)M 2,4-D at 26°C in the dark optimized the expression of differential resistance when inoculated with hyphae of P. cinnamomi. High concentration of 2,4-D (5×10(-5)M) inhibited growth of P. cinnamomi.
ISSN:	0721-7714 1432-203X
DOI:	10.1007/BF00232102