Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins

The two flexible rod-like flagella (∼500 nm in diameter and 5-15 μm long) of Chlamydomonas reinhardtii, a unicellular green alga, can weakly align molecules in an external magnetic field, thereby enabling the measurement of various residual dipolar couplings in solution NMR spectroscopy.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2013-12, Vol.49 (97), p.11403-11405
Hauptverfasser:	Singh, Himanshu, Shukla, Manish, Rao, Basuthkar J, Chary, Kandala V R
Format:	Artikel
Sprache:	eng
Schlagworte:	Chlamydomonas reinhardtii - physiology Flagella - metabolism Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Nitrogen Isotopes - chemistry Plant Proteins - chemistry Plant Proteins - metabolism Ubiquitin - chemistry Ubiquitin - metabolism
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Beschreibung
Zusammenfassung:	The two flexible rod-like flagella (∼500 nm in diameter and 5-15 μm long) of Chlamydomonas reinhardtii, a unicellular green alga, can weakly align molecules in an external magnetic field, thereby enabling the measurement of various residual dipolar couplings in solution NMR spectroscopy.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c3cc46233a