Oligonucleotide-directed site-specific mutagenesis of the lac permease of Escherichia coli

In order to determine the role of amino acid residues of sequences in the function of a particular protein or enzyme, it is helpful to be able to make specific alterations in the protein in a highly precise fashion. With the recent advent of site-directed mutagenesis, this goal can be realized, and...

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Veröffentlicht in:	Methods in enzymology 1986, Vol.125, p.214-230
Hauptverfasser:	Sarkar, H K, Viitanen, P V, Padan, E, Trumble, W R, Poonian, M S, McComas, W, Kaback, H R
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Coliphages - genetics Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Proteins Genetic Vectors lactose permease Membrane Transport Proteins - genetics Monosaccharide Transport Proteins Mutation Nucleic Acid Hybridization Oligodeoxyribonucleotides - pharmacology Plasmids reviews site-directed mutagenesis Species Specificity Symporters Transfection
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Beschreibung
Zusammenfassung:	In order to determine the role of amino acid residues of sequences in the function of a particular protein or enzyme, it is helpful to be able to make specific alterations in the protein in a highly precise fashion. With the recent advent of site-directed mutagenesis, this goal can be realized, and it is the purpose of this article to describe an application of the technique to the lac permease of Escherichia coli .
ISSN:	0076-6879