Synthesis of eicosapentaenoyl phosphatidylcholines by polyethylene glycol-modified lipase in benzene

Lipase from Candida cylindracea was modified with activated polyethylene glycol, which was synthesized from alpha -carboxymethyl- omega -methoxypoly-(Oxyethylene) and N-hydroxysuccinimide with dicyclohexylcarbodiimide. The modified lipase was soluble in organic solvents such as benzene and catalyzed...

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Veröffentlicht in:Biotechnology letters 1986-01, Vol.8 (11), p.771-776
Hauptverfasser: YOSHIMOTO, T, NAKATA, M, YAMAGUCHI, S, FUNADA, T, SAITO, Y, INADA, Y
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Sprache:eng
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Zusammenfassung:Lipase from Candida cylindracea was modified with activated polyethylene glycol, which was synthesized from alpha -carboxymethyl- omega -methoxypoly-(Oxyethylene) and N-hydroxysuccinimide with dicyclohexylcarbodiimide. The modified lipase was soluble in organic solvents such as benzene and catalyzed the reaction of ester-exchange to synthesize eicosapentaenoyl phosphatidylcholines from dipalmitoyl phosphatidylcholine and eicosapentaenoic acid. Either one of palmitic acid at C-1 or C-2 position of the phosphatidylcholine was exchanged with eicosapentaenoic acid.
ISSN:0141-5492
1573-6776
DOI:10.1007/bf01020820