Role of rat liver inducible enzymes in in vitro metabolic transformation of 1-nitropyrene

Liver enzymes (S9) of rats induced with various drugs differently affected the mutagenic response of 1-nitropyrene (1-NP) in vitro. The role of inducible enzymes in 1-NP conversion was evaluated by high-performance liquid chromatography (HPLC) analyses of 1-NP metabolites formed during incubation in...

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Veröffentlicht in:	Toxicology letters 1986-07, Vol.32 (1), p.89-96
Hauptverfasser:	Belisario, M.A., Carrano, L., de Giulio, A., Buonocore, V.
Format:	Artikel
Sprache:	eng
Schlagworte:	1-nitropyrene Animals Biological and medical sciences Biotransformation Chemical mutagenesis Cytochrome P-450 Enzyme System - physiology Enzyme Induction Hydroxylation In Vitro Techniques liver Liver - enzymology Male Medical sciences MFO-inducers Mutagens - metabolism Pyrenes - metabolism Rat Rats Rats, Inbred Strains Salmonella Toxicology
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Zusammenfassung:	Liver enzymes (S9) of rats induced with various drugs differently affected the mutagenic response of 1-nitropyrene (1-NP) in vitro. The role of inducible enzymes in 1-NP conversion was evaluated by high-performance liquid chromatography (HPLC) analyses of 1-NP metabolites formed during incubation in vitro of the chemical with each S9 fraction. Methylcholanthrene (MC)-induced S9 preferentially results in 1-NP hydroxylation in position 6 or 8. Metabolic interconversions among 1-NP metabolites could be inferred from the HPLC patterns.
ISSN:	0378-4274 1879-3169
DOI:	10.1016/0378-4274(86)90053-6