Genetic transformation in the grain legume Cicer arietinum L. (chickpea)

In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cu...

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Veröffentlicht in:	Plant cell reports 1993-02, Vol.12 (4), p.194-198
Hauptverfasser:	FONTANA, G. S, SANTINI, L, CARETTO, S, FRUGIS, G, MARIOTTI, D
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Transgenic animals and transgenic plants Transgenic plants
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase II and ß-glucuronidase activities and the presence in their genome of integrated bacterial DNA.
ISSN:	0721-7714 1432-203X
DOI:	10.1007/BF00237052