Identification and production of flavonoids in cell suspension culture of Scutellaria baicalensis G

A high yielding line of Scutellaria baicalensis G. has successfully been developed to produce flavonoids. Major components of the flavonoids were identified as baicalin and wogonin-7-O-glucuronic acid by a series of instrumental analyses using UV, IR, MASS, and NMR. After 12 days in suspension culture, the cell growth reached 14 g(DW)/l, and baicalin and wogonin-7-O-glucuronic acid were obtained in concentrations of 2.9 g/l, and 1.07 g/l, respectively. The culture temperature was found to be an important parameter for improving production yield of the flavonoids. The yield of baicalin was observed to increase to 4.2 g/l by shifting the temperature from 30 degrees C to 25 degrees C after of suspension culture.