RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing?

Highlights • Cas9 functions as an RNA-directed double-stranded DNA endonuclease. • Cas9 is easily programmable using customizable RNAs. • Cas9 is a promising tool in the genome editing toolbox.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Trends in microbiology (Regular ed.) 2013-11, Vol.21 (11), p.562-567
Hauptverfasser:	Gasiunas, Giedrius, Siksnys, Virginijus
Format:	Artikel
Sprache:	eng
Schlagworte:	clustered regularly interspaced short palindromic repeat (CRISPR) Clustered Regularly Interspaced Short Palindromic Repeats Deoxyribonuclease I - metabolism DNA DNA - metabolism gene targeting Genetic Engineering - methods genome Internal Medicine Molecular Biology - methods molecular tools nucleases protein engineering Recombinant Proteins - genetics Recombinant Proteins - metabolism Recombination, Genetic RNA RNA - metabolism RNA-guided DNA cleavage surgery zinc finger motif
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Highlights • Cas9 functions as an RNA-directed double-stranded DNA endonuclease. • Cas9 is easily programmable using customizable RNAs. • Cas9 is a promising tool in the genome editing toolbox.
ISSN:	0966-842X 1878-4380
DOI:	10.1016/j.tim.2013.09.001