PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality

Good or poor wheat bread-making quality is associated with two allelic pairs at the Glu-D1 complex locus, designated 1Dx5-1Dy1O and 1Dx2-1Dy12, respectively. The polymerase chain reaction (PCR) verified the presence of the HMW-glutenin 1Dx5 gene from genomic DNA extracted from part of the endosperm...

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Veröffentlicht in:	Theoretical and applied genetics 1994-08, Vol.88 (6-7), p.759-763
Hauptverfasser:	Ovidio, R. de, Anderson, O.D. (Universita degli Studi della Tuscia, Viterbo (Italy). Dept. di Agrobiologia e Agrochimica)
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Sprache:	eng
Schlagworte:	1dx2 gene 1dx5 gene 1dy10 gene 1dy12 gene alleles baking quality breadmaking CALIDAD COCCION AL HORNO complex loci CUISSON AU FOUR GENE GENES glu-d1 locus GLUTENINAS GLUTENINE glutenins LOCI LOCUS multigene family polymerase chain reaction QUALITE TRITICUM AESTIVUM
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container_title	Theoretical and applied genetics
container_volume	88
creator	Ovidio, R. de Anderson, O.D. (Universita degli Studi della Tuscia, Viterbo (Italy). Dept. di Agrobiologia e Agrochimica)
description	Good or poor wheat bread-making quality is associated with two allelic pairs at the Glu-D1 complex locus, designated 1Dx5-1Dy1O and 1Dx2-1Dy12, respectively. The polymerase chain reaction (PCR) verified the presence of the HMW-glutenin 1Dx5 gene from genomic DNA extracted from part of the endosperm of a single dry seed, or a small amount of leaf or root tissue, of several bread-wheat cultivars. This easy, quick, and non-destructive PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting bread-wheat genotypes with good bread-making properties.
doi_str_mv	10.1007/BF01253982
format	Article
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(Universita degli Studi della Tuscia, Viterbo (Italy). Dept. di Agrobiologia e Agrochimica)</creatorcontrib><title>PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>Good or poor wheat bread-making quality is associated with two allelic pairs at the Glu-D1 complex locus, designated 1Dx5-1Dy1O and 1Dx2-1Dy12, respectively. The polymerase chain reaction (PCR) verified the presence of the HMW-glutenin 1Dx5 gene from genomic DNA extracted from part of the endosperm of a single dry seed, or a small amount of leaf or root tissue, of several bread-wheat cultivars. 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subjects	1dx2 gene 1dx5 gene 1dy10 gene 1dy12 gene alleles baking quality breadmaking CALIDAD COCCION AL HORNO complex loci CUISSON AU FOUR GENE GENES glu-d1 locus GLUTENINAS GLUTENINE glutenins LOCI LOCUS multigene family polymerase chain reaction QUALITE TRITICUM AESTIVUM
title	PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality
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