PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality

Good or poor wheat bread-making quality is associated with two allelic pairs at the Glu-D1 complex locus, designated 1Dx5-1Dy1O and 1Dx2-1Dy12, respectively. The polymerase chain reaction (PCR) verified the presence of the HMW-glutenin 1Dx5 gene from genomic DNA extracted from part of the endosperm of a single dry seed, or a small amount of leaf or root tissue, of several bread-wheat cultivars. This easy, quick, and non-destructive PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting bread-wheat genotypes with good bread-making properties.