In-Plane Parallel Scanning: A Microarray Technology for Point-of-Care Testing
A new microarray technology is described for rapid, inexpensive, multiplex diagnostics assays. Referred to as “in-plane parallel scanning” (IPPS), this technology replaces expensive laser scanning with a grid of 100-μm-wide waveguides embedded in the chip’s substrate, enabling real-time quantificati...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2010-11, Vol.82 (21), p.8856-8865 |
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| creator | Duer, Reuven Lund, Russell Tanaka, Richard Christensen, Douglas A Herron, James N |
| description | A new microarray technology is described for rapid, inexpensive, multiplex diagnostics assays. Referred to as “in-plane parallel scanning” (IPPS), this technology replaces expensive laser scanning with a grid of 100-μm-wide waveguides embedded in the chip’s substrate, enabling real-time quantification of molecular complex formation on the chip’s surface. Compared to conventional microarray technology, IPPS has advantages of shorter assay time and lower instrument cost and complexity so that the platform can potentially be used in point-of-care (POC) settings. Two different chip formats are described: a low-density microarray with 10 sensing wells (IPPS-10) and a medium-density one with 100 sensing wells (IPPS-100). Performance was evaluated in two different proof-of-principle immunoassays: interleukin-1β (IL-1β) and Clostridium difficile toxin A. The two assays gave similar limits of detection of 0.67 and 0.94 pM, respectively. A saturation kinetics model described the sensor response with apparent dissociation constants of 511 pM for IL-1β and 6.47 nM for C. difficile toxin A toxoid. The multiplexing capabilities of the IPPS technology were also demonstrated in a multiplex assay for both analytes on the same IPPS-10 chip. Based on these results, the IPPS technology holds promise for translating diagnostic microarrays into near-patient environments. |
| doi_str_mv | 10.1021/ac101571b |
| format | Article |
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Referred to as “in-plane parallel scanning” (IPPS), this technology replaces expensive laser scanning with a grid of 100-μm-wide waveguides embedded in the chip’s substrate, enabling real-time quantification of molecular complex formation on the chip’s surface. Compared to conventional microarray technology, IPPS has advantages of shorter assay time and lower instrument cost and complexity so that the platform can potentially be used in point-of-care (POC) settings. Two different chip formats are described: a low-density microarray with 10 sensing wells (IPPS-10) and a medium-density one with 100 sensing wells (IPPS-100). Performance was evaluated in two different proof-of-principle immunoassays: interleukin-1β (IL-1β) and Clostridium difficile toxin A. The two assays gave similar limits of detection of 0.67 and 0.94 pM, respectively. A saturation kinetics model described the sensor response with apparent dissociation constants of 511 pM for IL-1β and 6.47 nM for C. difficile toxin A toxoid. The multiplexing capabilities of the IPPS technology were also demonstrated in a multiplex assay for both analytes on the same IPPS-10 chip. Based on these results, the IPPS technology holds promise for translating diagnostic microarrays into near-patient environments.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac101571b</identifier><identifier>PMID: 20945920</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Applied sciences ; Bacteria ; Bacterial Toxins - analysis ; Chemistry ; Clostridium difficile - isolation & purification ; Cytokines ; Diagnostics ; Enterocolitis, Pseudomembranous - diagnosis ; Enterotoxins - analysis ; Equipment Design ; Exact sciences and technology ; General, instrumentation ; Global environmental pollution ; Humans ; Interleukin-1beta - analysis ; Limit of Detection ; Measurement ; Microarray Analysis - instrumentation ; Miscellaneous ; Point-of-Care Systems ; Pollution ; Reaction kinetics</subject><ispartof>Analytical chemistry (Washington), 2010-11, Vol.82 (21), p.8856-8865</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Nov 1, 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a372t-4b9b048f8eb231cd7716a09f112a9508879c8a0f03ebe5c24f982607030bcab33</citedby><cites>FETCH-LOGICAL-a372t-4b9b048f8eb231cd7716a09f112a9508879c8a0f03ebe5c24f982607030bcab33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac101571b$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac101571b$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23382425$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20945920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duer, Reuven</creatorcontrib><creatorcontrib>Lund, Russell</creatorcontrib><creatorcontrib>Tanaka, Richard</creatorcontrib><creatorcontrib>Christensen, Douglas A</creatorcontrib><creatorcontrib>Herron, James N</creatorcontrib><title>In-Plane Parallel Scanning: A Microarray Technology for Point-of-Care Testing</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A new microarray technology is described for rapid, inexpensive, multiplex diagnostics assays. Referred to as “in-plane parallel scanning” (IPPS), this technology replaces expensive laser scanning with a grid of 100-μm-wide waveguides embedded in the chip’s substrate, enabling real-time quantification of molecular complex formation on the chip’s surface. Compared to conventional microarray technology, IPPS has advantages of shorter assay time and lower instrument cost and complexity so that the platform can potentially be used in point-of-care (POC) settings. Two different chip formats are described: a low-density microarray with 10 sensing wells (IPPS-10) and a medium-density one with 100 sensing wells (IPPS-100). Performance was evaluated in two different proof-of-principle immunoassays: interleukin-1β (IL-1β) and Clostridium difficile toxin A. The two assays gave similar limits of detection of 0.67 and 0.94 pM, respectively. A saturation kinetics model described the sensor response with apparent dissociation constants of 511 pM for IL-1β and 6.47 nM for C. difficile toxin A toxoid. The multiplexing capabilities of the IPPS technology were also demonstrated in a multiplex assay for both analytes on the same IPPS-10 chip. Based on these results, the IPPS technology holds promise for translating diagnostic microarrays into near-patient environments.</description><subject>Analytical chemistry</subject><subject>Applied sciences</subject><subject>Bacteria</subject><subject>Bacterial Toxins - analysis</subject><subject>Chemistry</subject><subject>Clostridium difficile - isolation & purification</subject><subject>Cytokines</subject><subject>Diagnostics</subject><subject>Enterocolitis, Pseudomembranous - diagnosis</subject><subject>Enterotoxins - analysis</subject><subject>Equipment Design</subject><subject>Exact sciences and technology</subject><subject>General, instrumentation</subject><subject>Global environmental pollution</subject><subject>Humans</subject><subject>Interleukin-1beta - analysis</subject><subject>Limit of Detection</subject><subject>Measurement</subject><subject>Microarray Analysis - instrumentation</subject><subject>Miscellaneous</subject><subject>Point-of-Care Systems</subject><subject>Pollution</subject><subject>Reaction kinetics</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0E1PGzEQBmCrKiqB9tA_gFaVKrWHpTNj766XWxSVgpSISMB5NWtsumhjg50c8u9rREokOPngZ75eIb4inCIQ_mKDgFWD_QcxwYqgrLWmj2ICALKkBuBQHKX0AIDZ1Z_EIUGrqpZgIhaXvlyO7G2x5MjjaMfi2rD3g78_K6bFYjAxcIy8LW6s-evDGO63hQuxWIbBr8vgyhlHmz_TOpd8FgeOx2S_7N5jcXv--2Z2Uc6v_lzOpvOSZUPrUvVtD0o7bXuSaO6aBmuG1iEStxVo3bRGMziQtreVIeVaTTU0IKE33Et5LH689H2M4WmTZ3erIRk7Ph8SNqlDpTShbogy_faGPoRN9Hm7TqPSoGrCjH6-oHxtStG67jEOK47bDqF7jrh7jTjbk13DTb-yd6_yf6YZfN8BToZHF9mbIe2dlJoUVXvHJu2Xej_wH9vci-0</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Duer, Reuven</creator><creator>Lund, Russell</creator><creator>Tanaka, Richard</creator><creator>Christensen, Douglas A</creator><creator>Herron, James N</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>In-Plane Parallel Scanning: A Microarray Technology for Point-of-Care Testing</title><author>Duer, Reuven ; Lund, Russell ; Tanaka, Richard ; Christensen, Douglas A ; Herron, James N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a372t-4b9b048f8eb231cd7716a09f112a9508879c8a0f03ebe5c24f982607030bcab33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytical chemistry</topic><topic>Applied sciences</topic><topic>Bacteria</topic><topic>Bacterial Toxins - analysis</topic><topic>Chemistry</topic><topic>Clostridium difficile - isolation & purification</topic><topic>Cytokines</topic><topic>Diagnostics</topic><topic>Enterocolitis, Pseudomembranous - diagnosis</topic><topic>Enterotoxins - analysis</topic><topic>Equipment Design</topic><topic>Exact sciences and technology</topic><topic>General, instrumentation</topic><topic>Global environmental pollution</topic><topic>Humans</topic><topic>Interleukin-1beta - analysis</topic><topic>Limit of Detection</topic><topic>Measurement</topic><topic>Microarray Analysis - instrumentation</topic><topic>Miscellaneous</topic><topic>Point-of-Care Systems</topic><topic>Pollution</topic><topic>Reaction kinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duer, Reuven</creatorcontrib><creatorcontrib>Lund, Russell</creatorcontrib><creatorcontrib>Tanaka, Richard</creatorcontrib><creatorcontrib>Christensen, Douglas A</creatorcontrib><creatorcontrib>Herron, James N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duer, Reuven</au><au>Lund, Russell</au><au>Tanaka, Richard</au><au>Christensen, Douglas A</au><au>Herron, James N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-Plane Parallel Scanning: A Microarray Technology for Point-of-Care Testing</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>82</volume><issue>21</issue><spage>8856</spage><epage>8865</epage><pages>8856-8865</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A new microarray technology is described for rapid, inexpensive, multiplex diagnostics assays. Referred to as “in-plane parallel scanning” (IPPS), this technology replaces expensive laser scanning with a grid of 100-μm-wide waveguides embedded in the chip’s substrate, enabling real-time quantification of molecular complex formation on the chip’s surface. Compared to conventional microarray technology, IPPS has advantages of shorter assay time and lower instrument cost and complexity so that the platform can potentially be used in point-of-care (POC) settings. Two different chip formats are described: a low-density microarray with 10 sensing wells (IPPS-10) and a medium-density one with 100 sensing wells (IPPS-100). 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| source | ACS Publications; MEDLINE |
| subjects | Analytical chemistry Applied sciences Bacteria Bacterial Toxins - analysis Chemistry Clostridium difficile - isolation & purification Cytokines Diagnostics Enterocolitis, Pseudomembranous - diagnosis Enterotoxins - analysis Equipment Design Exact sciences and technology General, instrumentation Global environmental pollution Humans Interleukin-1beta - analysis Limit of Detection Measurement Microarray Analysis - instrumentation Miscellaneous Point-of-Care Systems Pollution Reaction kinetics |
| title | In-Plane Parallel Scanning: A Microarray Technology for Point-of-Care Testing |
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