Use of lectin affinity chromatography for the purification of collagenase from human polymorphonuclear leukocytes

Polymorphonuclear leukocytes (PMNLs) store collagenase in an inactive form in secretory granules. The enzyme can be activated in vitro by limited proteolysis or by sulfhydryl-modifying agents such as N-ethylmaleimide (NEM). We have enriched NEM-activated collagenase 820-fold using granule isolation,...

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Veröffentlicht in:Biochemistry (Easton) 1986-08, Vol.25 (17), p.4757-4762
Hauptverfasser: Callaway, James E, Garcia, Joseph A, Hersh, Carol Lipsey, Yeh, Raymond K, Gilmore-Hebert, Maureen
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Sprache:eng
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