Effect of toxin from the cyanobacterium Microcystis aeruginosa on ultrastructural morphology and actin polymerization in isolated hepatocytes

Freshly isolated rat hepatocytes incubated with the hepatotoxin from the cyanobacterium Microcystis aeruginosa are rapidly deformed (blebbed). Transmission electron microscopy shows the appearance of unusual intracellular structures and rearrangement of cellular organelles, without any change in the polymerization state of actin. Cytochalasin E (20 μM), a fungal metabolite that causes blebbing of hepatocytes, had no significant effect on the polymerization state of cellular actin, but if Microcystis toxin (10 μM) was added together with cytochalasin E (20 μM), there was a significant increase (from 30% to 44%) in the proportion of unpolymerized G-actin in hepatocytes. These findings are in contrast to the effect of phalloidin (12.5 – 37.5 μM), a peptide hepatotoxin from the poisonous mushroom Amanita phalloides, which also causes blebbing of hepatocytes, and was shown in this study to decrease the level of unpolymerized G-actin in the cells to below measurable levels when added by itself or together with Microcystis toxin or cytochalasin E.