Mechanisms of Ethylenediurea (EDU) Induced Ozone Protection: Reexamination of Free Radical Scavenger Systems in Snap Bean Exposed to O sub(3)

Tests have proven that ethylenediurea (EDU) is capable of protecting leaf tissues from damage resulting from ozone exposure. Research conducted to evaluate the hypothesis that EDU protects against this type of damage by scavenging hydroxyl free radicals is described. Tests carried out in vitro utilized a high-performance liquid chromatography system fitted with an electrochemical detector. These tests suggested that EDU does not act as an antioxidant for the removal of hydroxyl free radicals. Experiments performed to evaluate the impacts of O sub(3) and EDU on leaf antioxidant scavenger systems are detailed. Researchers analyzed these processes in samples of the O sub(3)-sensitive snap bean (Phaseolus vulgaris cv. Bush Blue Lake 290). Potential mechanisms for the protective effects of EDU are investigated.