An assay combining cell culture with reverse transcriptase PCR to detect and determine the infectivity of waterborne Cryptosporidium parvum

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Veröffentlicht in:	Applied and Environmental Microbiology 1997-05, Vol.63 (5), p.2029-2037
Hauptverfasser:	ROCHELLE, P. A, FERGUSON, D. M, HANDOJO, T. J, DE LEON, R, STEWART, M. H, WOLFE, R. L
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Protozoan - immunology Biological and medical sciences Caco-2 Cells Cell Culture Techniques - methods Cells, Cultured Cryptosporidiosis - immunology Cryptosporidiosis - parasitology Cryptosporidium parvum Cryptosporidium parvum - immunology Cryptosporidium parvum - isolation & purification DNA, Protozoan - analysis DNA, Protozoan - isolation & purification Drinking water Electrophoresis, Agar Gel Fluorescent Antibody Technique, Direct Fundamental and applied biological sciences. Psychology Fungi General aspects and techniques HSP70 Heat-Shock Proteins - genetics Humans Microbiology Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotides - genetics Polymerase Chain Reaction - methods Protozoa RNA, Protozoan - genetics Sensitivity and Specificity Tests Water Microbiology Water pollution
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Zusammenfassung:	Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: AEM
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.63.5.2029-2037.1997