Fungal fimbriae. V. Protein A+gold immunocytochemical labeling of the fimbriae of Ustilago violacea

Protein A-gold immunocytochemical labeling of long protein appendages (fimbriae) on the surfaces of cells of Ustilago violacea is described. The specificity of the technique is confirmed by a series of serological and morphological controls. The technique allows specific identification and localization of fimbrial antigens in electron microscopic studies of a variety of fungi in both pathogenic and saprophytic situations.