Contrasting of Biological Samples for X-Ray Synchrotron Microtomography

The method of contrasting with iodine ions was developed to obtain high-resolution 3D images of large biological specimens using a synchrotron X-ray microtomography unit. It was shown that the samples (late mouse embryos) treated with 50% Lugol solution with addition of 25% ethanol for 48 h followed...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2013-08, Vol.155 (4), p.413-416
Hauptverfasser:	Efimova, O. I., Khlebnikov, A. S., Senin, R. A., Voronin, P. A., Anokhin, K. V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biomedical and Life Sciences Biomedicine Brain - diagnostic imaging Cell Biology Contrast Media Embryo, Mammalian - diagnostic imaging Female Internal Medicine Iodides Laboratory Medicine Male Mice Mice, Inbred C57BL Nuclear radiation Particle accelerators Pathology Phosphates Physiology X-Ray Microtomography - methods
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Beschreibung
Zusammenfassung:	The method of contrasting with iodine ions was developed to obtain high-resolution 3D images of large biological specimens using a synchrotron X-ray microtomography unit. It was shown that the samples (late mouse embryos) treated with 50% Lugol solution with addition of 25% ethanol for 48 h followed by a 48-h washout in phosphate buffered saline had maximum contrast and lowest compression artifacts. Processing of samples by this protocol allowed detecting zones of active proliferation. Incubation of brain samples for 120 h in 7.6% meglumine/sodium diatrizoate without washout ensured the best contrast during myelin identifi cation.
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-013-2165-6