A luminol chemiluminescence method for sensing histidine and lysine using enzyme reactions

The analysis of free amino acids in urine and plasma is useful for estimating disease status in clinical diagnoses. Changes in the concentration of free amino acids in foods are also useful markers of freshness, nutrition, and taste. In this study, the specific interaction between aminoacyl–tRNA syn...

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Veröffentlicht in:	Analytical biochemistry 2013-12, Vol.443 (1), p.22-26
Hauptverfasser:	Kugimiya, Akimitsu, Fukada, Rie, Funamoto, Daiki
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acid Amino Acyl-tRNA Synthetases - chemistry aminoacyl tRNA ligases Aminoacyl–tRNA synthetase Biosensing Calibration chemiluminescence Diphosphates - chemistry enzymatic reactions Enzyme Assays Enzyme reaction foods free amino acids freshness histidine Histidine - analysis Hydrogen Peroxide - chemistry Inorganic Pyrophosphatase - chemistry Limit of Detection Luminescence Luminescent Measurements Luminol - chemistry Luminol chemiluminescence lysine Lysine - analysis nutrition Protein Binding Pyruvate Oxidase - chemistry Reproducibility of Results Solutions taste Temperature urine
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Beschreibung
Zusammenfassung:	The analysis of free amino acids in urine and plasma is useful for estimating disease status in clinical diagnoses. Changes in the concentration of free amino acids in foods are also useful markers of freshness, nutrition, and taste. In this study, the specific interaction between aminoacyl–tRNA synthetase (aaRS) and its corresponding amino acid was used to measure amino acid concentrations. Pyrophosphate released by the amino acid–aaRS binding reaction was detected by luminol chemiluminescence; the method provided selective quantitation of 1.0–30μM histidine and 1.0–60μM lysine.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2013.08.012