Identification of antimony resistance markers in Leishmania tropica field isolates through a cDNA-AFLP approach

•Differential gene expression in resistant and sensitive L. tropica by cDNA-AFLP.•The expression of detected genes was confirmed using real time RT-PCR.•MRPA, PTP and PGK genes were upregulated in resistant isolate (P value

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Bibliographische Detailangaben
Veröffentlicht in:	Experimental parasitology 2013-10, Vol.135 (2), p.344-349
Hauptverfasser:	Kazemi-Rad, Elham, Mohebali, Mehdi, Khadem-Erfan, Mohammad Bagher, Saffari, Mojtaba, Raoofian, Reza, Hajjaran, Homa, Hadighi, Ramtin, Khamesipour, Ali, Rezaie, Sassan, Abedkhojasteh, Hoda, Heidari, Mansour
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplified Fragment Length Polymorphism Analysis antimony Antimony - pharmacology Antimony resistance Antiprotozoal Agents - pharmacology cDNA-AFLP Cell Line cutaneous leishmaniasis DNA Fragmentation DNA, Complementary - chemistry DNA, Complementary - metabolism DNA, Protozoan - chemistry DNA, Protozoan - metabolism drug resistance Drug Resistance - genetics drugs gene expression regulation genes Genes, Protozoan glycolysis Inhibitory Concentration 50 Leishmania tropica Leishmania tropica - drug effects Leishmania tropica - genetics Meglumine - pharmacology mitogen-activated protein kinase Organometallic Compounds - pharmacology Parasitic Sensitivity Tests parasitology phosphoglycerate kinase phosphorylation protein-tyrosine-phosphatase Real-Time Polymerase Chain Reaction Real-time RT-PCR reverse transcriptase polymerase chain reaction RNA, Protozoan - genetics RNA, Protozoan - isolation & purification therapeutics
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Beschreibung
Zusammenfassung:	•Differential gene expression in resistant and sensitive L. tropica by cDNA-AFLP.•The expression of detected genes was confirmed using real time RT-PCR.•MRPA, PTP and PGK genes were upregulated in resistant isolate (P value
ISSN:	0014-4894 1090-2449
DOI:	10.1016/j.exppara.2013.07.018