Duct ELISA for detection of bacteria in fluid samples

Tygon ducts were used as matrix in an enzyme-linked immunosorbent assay for detection of bacteria in fluid samples. Dilute water samples of Francisella tularensis organisms were pumped through antibody-coated ducts, thereby enriching bacterial antigen at the duct wall. The assay was completed in the...

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Veröffentlicht in:	Journal of microbiological methods 1986, Vol.5 (1), p.41-47
Hauptverfasser:	SANDSTROM, G. E, WOLF-WATZ, H, TARNVIK, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences ELISA Francisella tularensis Fundamental and applied biological sciences. Psychology Microbiology Salmonella typhimurium Water
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Zusammenfassung:	Tygon ducts were used as matrix in an enzyme-linked immunosorbent assay for detection of bacteria in fluid samples. Dilute water samples of Francisella tularensis organisms were pumped through antibody-coated ducts, thereby enriching bacterial antigen at the duct wall. The assay was completed in the duct. The possibility of detecting F. tularensis organisms was only slightly disturbed by the concomitant presence of heterologous bacteria. The technique was also applied in the detection of Salmonella typhimurium in water samples.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/0167-7012(86)90022-9