Rapid analysis of amino acids using pre-column derivatization
A new approach to the pre-column derivatization and analysis of amino acids is described. The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino a...
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| Veröffentlicht in: | Journal of Chromatography A 1984-01, Vol.336 (1), p.93-104 |
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| container_end_page | 104 |
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| container_issue | 1 |
| container_start_page | 93 |
| container_title | Journal of Chromatography A |
| container_volume | 336 |
| creator | Bidlingmeyer, Brian A. Cohen, Steven A. Tarvin, Thomas L. |
| description | A new approach to the pre-column derivatization and analysis of amino acids is described. The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydorlyzates. The liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids with 12-min analysis time and a 1-pmol sensitivity. |
| doi_str_mv | 10.1016/S0378-4347(00)85133-6 |
| format | Article |
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The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydorlyzates. The liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids with 12-min analysis time and a 1-pmol sensitivity.</description><identifier>ISSN: 0378-4347</identifier><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0378-4347(00)85133-6</identifier><identifier>PMID: 6396315</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>amino acids ; Amino Acids - analysis ; Applied sciences ; Chemical Phenomena ; Chemistry ; Drug Stability ; Exact sciences and technology ; Humans ; Hydrolysis ; Insulin - analysis ; Isothiocyanates ; liquid chromatography ; Other techniques and industries ; Oxytocin - analysis ; Peptides - analysis ; Proteins - analysis ; Spectrophotometry, Ultraviolet - methods ; Thiocyanates ; Trypsin - analysis</subject><ispartof>Journal of Chromatography A, 1984-01, Vol.336 (1), p.93-104</ispartof><rights>1984</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-2019e12c7e743d23633c90a2d75627c29f6591068cc424889800727a9596dda3</citedby><cites>FETCH-LOGICAL-c507t-2019e12c7e743d23633c90a2d75627c29f6591068cc424889800727a9596dda3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23910,23911,25119,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7889755$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6396315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bidlingmeyer, Brian A.</creatorcontrib><creatorcontrib>Cohen, Steven A.</creatorcontrib><creatorcontrib>Tarvin, Thomas L.</creatorcontrib><title>Rapid analysis of amino acids using pre-column derivatization</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr</addtitle><description>A new approach to the pre-column derivatization and analysis of amino acids is described. The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydorlyzates. The liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids with 12-min analysis time and a 1-pmol sensitivity.</description><subject>amino acids</subject><subject>Amino Acids - analysis</subject><subject>Applied sciences</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Drug Stability</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Insulin - analysis</subject><subject>Isothiocyanates</subject><subject>liquid chromatography</subject><subject>Other techniques and industries</subject><subject>Oxytocin - analysis</subject><subject>Peptides - analysis</subject><subject>Proteins - analysis</subject><subject>Spectrophotometry, Ultraviolet - methods</subject><subject>Thiocyanates</subject><subject>Trypsin - analysis</subject><issn>0378-4347</issn><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQgHNQaq3-hMIeRPSwOkk2yeYgIsUXFATtPcQkK5F9mewW6q932y69ehgGZr6ZTD6E5hhuMGB--wFU5GlGM3EFcJ0zTGnKj9D0UD5BpzF-A9AMCJmgCaeSU8ym6O5dt94mutblJvqYNEWiK183iTbexqSPvv5K2uBS05R9VSfWBb_Wnf8doqnP0HGhy-jOxzxDq6fH1eIlXb49vy4elqlhILqUAJYOEyOcyKgllFNqJGhiBeNEGCILziQGnhuTkSzPZQ4giNCSSW6tpjN0uV_bhuand7FTlY_GlaWuXdNHhbMM8uGvA8j2oAlNjMEVqg2-0mGjMKitKbUzpbZKFIDamVJ8mJuPD_SflbOHqVHT0L8Y-zoaXRZB18bHAyaGkwXbYvd7zA0u1t4FFY13tXHWB2c6ZRv_zyF_Jm2FEg</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Bidlingmeyer, Brian A.</creator><creator>Cohen, Steven A.</creator><creator>Tarvin, Thomas L.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19840101</creationdate><title>Rapid analysis of amino acids using pre-column derivatization</title><author>Bidlingmeyer, Brian A. ; Cohen, Steven A. ; Tarvin, Thomas L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-2019e12c7e743d23633c90a2d75627c29f6591068cc424889800727a9596dda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>amino acids</topic><topic>Amino Acids - analysis</topic><topic>Applied sciences</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Drug Stability</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Insulin - analysis</topic><topic>Isothiocyanates</topic><topic>liquid chromatography</topic><topic>Other techniques and industries</topic><topic>Oxytocin - analysis</topic><topic>Peptides - analysis</topic><topic>Proteins - analysis</topic><topic>Spectrophotometry, Ultraviolet - methods</topic><topic>Thiocyanates</topic><topic>Trypsin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bidlingmeyer, Brian A.</creatorcontrib><creatorcontrib>Cohen, Steven A.</creatorcontrib><creatorcontrib>Tarvin, Thomas L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bidlingmeyer, Brian A.</au><au>Cohen, Steven A.</au><au>Tarvin, Thomas L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid analysis of amino acids using pre-column derivatization</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>336</volume><issue>1</issue><spage>93</spage><epage>104</epage><pages>93-104</pages><issn>0378-4347</issn><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A new approach to the pre-column derivatization and analysis of amino acids is described. The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydorlyzates. The liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids with 12-min analysis time and a 1-pmol sensitivity.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>6396315</pmid><doi>10.1016/S0378-4347(00)85133-6</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0378-4347 |
| ispartof | Journal of Chromatography A, 1984-01, Vol.336 (1), p.93-104 |
| issn | 0378-4347 0021-9673 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Alma/SFX Local Collection |
| subjects | amino acids Amino Acids - analysis Applied sciences Chemical Phenomena Chemistry Drug Stability Exact sciences and technology Humans Hydrolysis Insulin - analysis Isothiocyanates liquid chromatography Other techniques and industries Oxytocin - analysis Peptides - analysis Proteins - analysis Spectrophotometry, Ultraviolet - methods Thiocyanates Trypsin - analysis |
| title | Rapid analysis of amino acids using pre-column derivatization |
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