Rapid analysis of amino acids using pre-column derivatization

A new approach to the pre-column derivatization and analysis of amino acids is described. The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino a...

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Veröffentlicht in:	Journal of Chromatography A 1984-01, Vol.336 (1), p.93-104
Hauptverfasser:	Bidlingmeyer, Brian A., Cohen, Steven A., Tarvin, Thomas L.
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acids Amino Acids - analysis Applied sciences Chemical Phenomena Chemistry Drug Stability Exact sciences and technology Humans Hydrolysis Insulin - analysis Isothiocyanates liquid chromatography Other techniques and industries Oxytocin - analysis Peptides - analysis Proteins - analysis Spectrophotometry, Ultraviolet - methods Thiocyanates Trypsin - analysis
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Beschreibung
Zusammenfassung:	A new approach to the pre-column derivatization and analysis of amino acids is described. The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydorlyzates. The liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids with 12-min analysis time and a 1-pmol sensitivity.
ISSN:	0378-4347 0021-9673
DOI:	10.1016/S0378-4347(00)85133-6