Phosphorylation of phospholipase B in the plasma membrane of Saccharomyces cerevisiae

Plasma membrane vesicles from Saccharomyces cerevisiae were incubated with [γ‐32P]ATP. Several phosphorylated protein bands were separated by LiDS polyacrylamide gel electrophoresis. One of these bands with an apparent Mr of 145 000 was identified by immunoprecipitation as a membrane‐bound phospholi...

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Veröffentlicht in:	FEMS microbiology letters 1985-01, Vol.30 (1/2), p.27-31
Hauptverfasser:	Witt, W, Wille, P, Fuhrmann, G.F
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology hydrolases immunoprecipitation LiDS polyacrylamide gel electrophoresis lysophospholipase Microbiology Mycology phosphorylation phosphorylation of membrane proteins plasma membrane plasma membranes protein A Saccharomyces cerevisiae Yeast
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Zusammenfassung:	Plasma membrane vesicles from Saccharomyces cerevisiae were incubated with [γ‐32P]ATP. Several phosphorylated protein bands were separated by LiDS polyacrylamide gel electrophoresis. One of these bands with an apparent Mr of 145 000 was identified by immunoprecipitation as a membrane‐bound phospholipase.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1985.tb00979.x