Restriction map and clone bank of tomato plastid DNA
Tomato plastid DNA has been isolated from leaf chloroplasts and PstI fragments cloned in the plasmid vector pUC8. Recombinant plasmids containing all but the largest PstI fragement were identified by colony hybridisation. A restriction map of the plastid DNA for four restriction endonucleases was ge...
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| Veröffentlicht in: | Current genetics 1985-01, Vol.10 (2), p.147-152 |
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| container_title | Current genetics |
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| creator | PHILLIPS, A. L |
| description | Tomato plastid DNA has been isolated from leaf chloroplasts and PstI fragments cloned in the plasmid vector pUC8. Recombinant plasmids containing all but the largest PstI fragement were identified by colony hybridisation. A restriction map of the plastid DNA for four restriction endonucleases was generated by digestion of the cloned PstI fragments and by hybridisation to Southern blots. The plastid DNA is a circular molecule of 156.6-159.4 kbp with a large inverted repeat, and shows high conservation of restriction sites with plastid DNA from tobacco and Petunia . |
| doi_str_mv | 10.1007/BF00636480 |
| format | Article |
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L</creatorcontrib><title>Restriction map and clone bank of tomato plastid DNA</title><title>Current genetics</title><description>Tomato plastid DNA has been isolated from leaf chloroplasts and PstI fragments cloned in the plasmid vector pUC8. Recombinant plasmids containing all but the largest PstI fragement were identified by colony hybridisation. A restriction map of the plastid DNA for four restriction endonucleases was generated by digestion of the cloned PstI fragments and by hybridisation to Southern blots. The plastid DNA is a circular molecule of 156.6-159.4 kbp with a large inverted repeat, and shows high conservation of restriction sites with plastid DNA from tobacco and Petunia .</description><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. 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L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restriction map and clone bank of tomato plastid DNA</atitle><jtitle>Current genetics</jtitle><date>1985-01-01</date><risdate>1985</risdate><volume>10</volume><issue>2</issue><spage>147</spage><epage>152</epage><pages>147-152</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><coden>CUGED5</coden><abstract>Tomato plastid DNA has been isolated from leaf chloroplasts and PstI fragments cloned in the plasmid vector pUC8. Recombinant plasmids containing all but the largest PstI fragement were identified by colony hybridisation. A restriction map of the plastid DNA for four restriction endonucleases was generated by digestion of the cloned PstI fragments and by hybridisation to Southern blots. The plastid DNA is a circular molecule of 156.6-159.4 kbp with a large inverted repeat, and shows high conservation of restriction sites with plastid DNA from tobacco and Petunia .</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/BF00636480</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Current genetics, 1985-01, Vol.10 (2), p.147-152 |
| issn | 0172-8083 1432-0983 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14399362 |
| source | SpringerNature Journals |
| subjects | Biological and medical sciences Fundamental and applied biological sciences. Psychology Genes. Genome Lycopersicon esculentum Molecular and cellular biology Molecular genetics |
| title | Restriction map and clone bank of tomato plastid DNA |
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