Restriction map and clone bank of tomato plastid DNA

Restriction map and clone bank of tomato plastid DNA

Tomato plastid DNA has been isolated from leaf chloroplasts and PstI fragments cloned in the plasmid vector pUC8. Recombinant plasmids containing all but the largest PstI fragement were identified by colony hybridisation. A restriction map of the plastid DNA for four restriction endonucleases was ge...

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Veröffentlicht in:Current genetics 1985-01, Vol.10 (2), p.147-152
1. Verfasser: PHILLIPS, A. L
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genes. Genome
Lycopersicon esculentum
Molecular and cellular biology
Molecular genetics
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Zusammenfassung:Tomato plastid DNA has been isolated from leaf chloroplasts and PstI fragments cloned in the plasmid vector pUC8. Recombinant plasmids containing all but the largest PstI fragement were identified by colony hybridisation. A restriction map of the plastid DNA for four restriction endonucleases was generated by digestion of the cloned PstI fragments and by hybridisation to Southern blots. The plastid DNA is a circular molecule of 156.6-159.4 kbp with a large inverted repeat, and shows high conservation of restriction sites with plastid DNA from tobacco and Petunia .
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00636480