Thin-layer chromatographic analysis of adriamycinone in fermentation broths

The authors report here a simple and rapid semiquantitative TLC method for adriamycin via adriamycin via adriamycinone in fermentation broths as a screening method without the need for special instrumentation. The method consists of a hydrolysis step, solvent extraction of the anthracyclinones from...

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Veröffentlicht in:	Journal of Chromatography A 1985, Vol.328, p.432-435
Hauptverfasser:	Dornberger, K., Stengel, C., Miosga, N.
Format:	Artikel
Sprache:	eng
Schlagworte:	adriamycinone Antibiotics Antibiotics (antibacterial agents, antifungal agents) Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents Applied microbiology Biological and medical sciences Biotechnology fermentation Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Microbiology Production of active biomolecules Streptomyces thin-layer chromatography
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Zusammenfassung:	The authors report here a simple and rapid semiquantitative TLC method for adriamycin via adriamycin via adriamycinone in fermentation broths as a screening method without the need for special instrumentation. The method consists of a hydrolysis step, solvent extraction of the anthracyclinones from the fermentation broths, development on a silica gel TLC sheet, silica gel-solvent transfer of the separated adriamycinone, and quantitation by fluorimetric assay.
ISSN:	0021-9673
DOI:	10.1016/S0021-9673(01)87423-9