MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE

MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE

The influence of culture conditions type of substrate and composition of culture medium on proliferation and hormone secretion in the newborn piglets adrenal primary cell culture was studied. The high level of proliferative activity accompanied by a monolayer and multicellular structures spheroids f...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biotechnologia acta 2012-09, Vol.5 (5), p.72-72
Hauptverfasser: Sidorenko, O S, Bozhok, G A, Bilyavskaya, S B, Ustichenko, V D, Gubina, N F, Kholodny, V S, Legach, Y I, Bondarenko, T P
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 72
container_issue 5
container_start_page 72
container_title Biotechnologia acta
container_volume 5
creator Sidorenko, O S
Bozhok, G A
Bilyavskaya, S B
Ustichenko, V D
Gubina, N F
Kholodny, V S
Legach, Y I
Bondarenko, T P
description The influence of culture conditions type of substrate and composition of culture medium on proliferation and hormone secretion in the newborn piglets adrenal primary cell culture was studied. The high level of proliferative activity accompanied by a monolayer and multicellular structures spheroids formation was observed when cultured in media supplemented with 10% fetal bovine serum. During further cultivation or subcultivation neuron-like cells migrated from spheroids. The process occurred in parallel with decreased cortisol secretion and did not depend on neuronal growth factor content in culture medium.
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1439237117</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3060955251</sourcerecordid><originalsourceid>FETCH-LOGICAL-p617-b828aba280df375edb5395d633d347800dc6dfa269601bb419393e54d145ddc63</originalsourceid><addsrcrecordid>eNpdzkFrgzAcBXAPG6x0_Q7CLrsISf6JMUeXxVbITNHIjkWbCCuuds38_pOtp53e4f14vLtohYVgCWPAH6JNCCeEEEZAGSWrqHwz9X5niraStjRVrmO5y-tcWlWXjS1lE5sirtT7i6mreF9utbJNnL_W6pcqrZtYttq2tXqM7oduDH5zy3VkC2XlLtFmW8pcJ5cU86TPSNb1HcmQG4Az73oGgrkUwAHlGULumLqhI6lIEe57igUI8Iw6TJlbOlhHz3-zl-v0Nfvwffj8CEc_jt3ZT3M4YAqCAMeYL_TpHz1N8_W8nFsUEZBRYBx-AKnkTnE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1429384357</pqid></control><display><type>article</type><title>MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Sidorenko, O S ; Bozhok, G A ; Bilyavskaya, S B ; Ustichenko, V D ; Gubina, N F ; Kholodny, V S ; Legach, Y I ; Bondarenko, T P</creator><creatorcontrib>Sidorenko, O S ; Bozhok, G A ; Bilyavskaya, S B ; Ustichenko, V D ; Gubina, N F ; Kholodny, V S ; Legach, Y I ; Bondarenko, T P</creatorcontrib><description>The influence of culture conditions type of substrate and composition of culture medium on proliferation and hormone secretion in the newborn piglets adrenal primary cell culture was studied. The high level of proliferative activity accompanied by a monolayer and multicellular structures spheroids formation was observed when cultured in media supplemented with 10% fetal bovine serum. During further cultivation or subcultivation neuron-like cells migrated from spheroids. The process occurred in parallel with decreased cortisol secretion and did not depend on neuronal growth factor content in culture medium.</description><identifier>ISSN: 1995-5537</identifier><language>eng</language><publisher>Kyiv: Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine</publisher><ispartof>Biotechnologia acta, 2012-09, Vol.5 (5), p.72-72</ispartof><rights>Copyright Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Sidorenko, O S</creatorcontrib><creatorcontrib>Bozhok, G A</creatorcontrib><creatorcontrib>Bilyavskaya, S B</creatorcontrib><creatorcontrib>Ustichenko, V D</creatorcontrib><creatorcontrib>Gubina, N F</creatorcontrib><creatorcontrib>Kholodny, V S</creatorcontrib><creatorcontrib>Legach, Y I</creatorcontrib><creatorcontrib>Bondarenko, T P</creatorcontrib><title>MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE</title><title>Biotechnologia acta</title><description>The influence of culture conditions type of substrate and composition of culture medium on proliferation and hormone secretion in the newborn piglets adrenal primary cell culture was studied. The high level of proliferative activity accompanied by a monolayer and multicellular structures spheroids formation was observed when cultured in media supplemented with 10% fetal bovine serum. During further cultivation or subcultivation neuron-like cells migrated from spheroids. The process occurred in parallel with decreased cortisol secretion and did not depend on neuronal growth factor content in culture medium.</description><issn>1995-5537</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdzkFrgzAcBXAPG6x0_Q7CLrsISf6JMUeXxVbITNHIjkWbCCuuds38_pOtp53e4f14vLtohYVgCWPAH6JNCCeEEEZAGSWrqHwz9X5niraStjRVrmO5y-tcWlWXjS1lE5sirtT7i6mreF9utbJNnL_W6pcqrZtYttq2tXqM7oduDH5zy3VkC2XlLtFmW8pcJ5cU86TPSNb1HcmQG4Az73oGgrkUwAHlGULumLqhI6lIEe57igUI8Iw6TJlbOlhHz3-zl-v0Nfvwffj8CEc_jt3ZT3M4YAqCAMeYL_TpHz1N8_W8nFsUEZBRYBx-AKnkTnE</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Sidorenko, O S</creator><creator>Bozhok, G A</creator><creator>Bilyavskaya, S B</creator><creator>Ustichenko, V D</creator><creator>Gubina, N F</creator><creator>Kholodny, V S</creator><creator>Legach, Y I</creator><creator>Bondarenko, T P</creator><general>Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine</general><scope>7QO</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20120901</creationdate><title>MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE</title><author>Sidorenko, O S ; Bozhok, G A ; Bilyavskaya, S B ; Ustichenko, V D ; Gubina, N F ; Kholodny, V S ; Legach, Y I ; Bondarenko, T P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p617-b828aba280df375edb5395d633d347800dc6dfa269601bb419393e54d145ddc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sidorenko, O S</creatorcontrib><creatorcontrib>Bozhok, G A</creatorcontrib><creatorcontrib>Bilyavskaya, S B</creatorcontrib><creatorcontrib>Ustichenko, V D</creatorcontrib><creatorcontrib>Gubina, N F</creatorcontrib><creatorcontrib>Kholodny, V S</creatorcontrib><creatorcontrib>Legach, Y I</creatorcontrib><creatorcontrib>Bondarenko, T P</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><jtitle>Biotechnologia acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sidorenko, O S</au><au>Bozhok, G A</au><au>Bilyavskaya, S B</au><au>Ustichenko, V D</au><au>Gubina, N F</au><au>Kholodny, V S</au><au>Legach, Y I</au><au>Bondarenko, T P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE</atitle><jtitle>Biotechnologia acta</jtitle><date>2012-09-01</date><risdate>2012</risdate><volume>5</volume><issue>5</issue><spage>72</spage><epage>72</epage><pages>72-72</pages><issn>1995-5537</issn><abstract>The influence of culture conditions type of substrate and composition of culture medium on proliferation and hormone secretion in the newborn piglets adrenal primary cell culture was studied. The high level of proliferative activity accompanied by a monolayer and multicellular structures spheroids formation was observed when cultured in media supplemented with 10% fetal bovine serum. During further cultivation or subcultivation neuron-like cells migrated from spheroids. The process occurred in parallel with decreased cortisol secretion and did not depend on neuronal growth factor content in culture medium.</abstract><cop>Kyiv</cop><pub>Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine</pub><tpages>1</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1995-5537
ispartof Biotechnologia acta, 2012-09, Vol.5 (5), p.72-72
issn 1995-5537
language eng
recordid cdi_proquest_miscellaneous_1439237117
source DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
title MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T06%3A07%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MORPHOFUNCTIONAL%20CHARACTERISTICS%20OF%20NEWBORN%20PIGLETS%20ADRENAL%20CELLS%20CULTURE&rft.jtitle=Biotechnologia%20acta&rft.au=Sidorenko,%20O%20S&rft.date=2012-09-01&rft.volume=5&rft.issue=5&rft.spage=72&rft.epage=72&rft.pages=72-72&rft.issn=1995-5537&rft_id=info:doi/&rft_dat=%3Cproquest%3E3060955251%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1429384357&rft_id=info:pmid/&rfr_iscdi=true