MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE

MORPHOFUNCTIONAL CHARACTERISTICS OF NEWBORN PIGLETS ADRENAL CELLS CULTURE

The influence of culture conditions type of substrate and composition of culture medium on proliferation and hormone secretion in the newborn piglets adrenal primary cell culture was studied. The high level of proliferative activity accompanied by a monolayer and multicellular structures spheroids f...

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Veröffentlicht in:Biotechnologia acta 2012-09, Vol.5 (5), p.72-72
Hauptverfasser: Sidorenko, O S, Bozhok, G A, Bilyavskaya, S B, Ustichenko, V D, Gubina, N F, Kholodny, V S, Legach, Y I, Bondarenko, T P
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Sprache:eng
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Zusammenfassung:The influence of culture conditions type of substrate and composition of culture medium on proliferation and hormone secretion in the newborn piglets adrenal primary cell culture was studied. The high level of proliferative activity accompanied by a monolayer and multicellular structures spheroids formation was observed when cultured in media supplemented with 10% fetal bovine serum. During further cultivation or subcultivation neuron-like cells migrated from spheroids. The process occurred in parallel with decreased cortisol secretion and did not depend on neuronal growth factor content in culture medium.
ISSN:1995-5537