IN VITRO ANTIBIOTIC SUSCEPTIBILITY OF GRAM POSITIVE ROD ISOLATED FROM SOIL IN THE VICINITY OF A DUMPING SITE

Aim: Antibiotics resistance of soil borne pathogens is a real problem for medical practitioners of India. In this investigation we have isolated a bacterium from the soil containing various types of dumps and observed its antibiotic susceptibility. Materials and Methods: The isolate found from the serial dilution of soil sample was named as Sample H and was taken for staining, antibiotic susceptibility test and 16S rDNA characterization. Results and Discussion: Gram staining showed it to be Gram positive rods and 16S rDNA characterization showed it to be Bacillus cereus strain KD125. The Phylogenetic analyses showed the Sample H similar to Bacillus anthracis strain APT25 (GenBank Accession Number: KC519402.1). The antibiotic sensitivity test showed that this Sample H is sensitive to Linezolid (LZ) and Ciprofloxacin (RC) and resistant to Cefixime (CF). It was also seen that combinations Linolenic acid (LI) increases the antibacterial activity of LZ. Whereas, Doxycycline (DOC) decreased the zone of inhibitions of Ampicillin (AMP) and Tinidazole (TI) did not show any change in antibacterial activity of RC. It was also seen that antacid Digene (DIG) slightly increased the affectivity of RC but decreased the antibacterial activity of LZ, whereas Pantoprazole (PAN) increased the antibacterial activity of RC and LZ. Thus, it was found that Sample H was maximum sensitive to the combination of LZ+ PAN followed by LZ+ LI and was resistant to all combinations of CF. Conclusion: Thus, sensitive antibiotics, effective combination of antibiotics or antacids obtain from this assay can help to treat this food poisoning causingpathogen Bacillus cereus efficiently.