Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae
ABSTRACT Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS‐related genes were distributed among the various serogroups and pulsed‐field gel electrophoresis of NotI‐digested genomes showed genetic div...
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| Veröffentlicht in: | Microbiology and immunology 2013-05, Vol.57 (5), p.334-339 |
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| creator | Morita, Masatomo Yamamoto, Shouji Hiyoshi, Hirotaka Kodama, Toshio Okura, Masatoshi Arakawa, Eiji Alam, Munirul Ohnishi, Makoto Izumiya, Hidemasa Watanabe, Haruo |
| description | ABSTRACT
Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS‐related genes were distributed among the various serogroups and pulsed‐field gel electrophoresis of NotI‐digested genomes showed genetic diversity in these strains. However, the restriction fragment length polymorphism profiles of the T3SS‐related genes had similar patterns. Additionally, naturally competent T3SS‐negative V. cholerae incorporated the ca. 47 kb gene cluster of T3SS, which had been integrated into a site on the chromosome by recombination. Therefore, it is suggested that horizontal gene transfer of T3SS‐related genes occurs among V. cholerae in natural ecosystems. |
| doi_str_mv | 10.1111/1348-0421.12039 |
| format | Article |
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Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS‐related genes were distributed among the various serogroups and pulsed‐field gel electrophoresis of NotI‐digested genomes showed genetic diversity in these strains. However, the restriction fragment length polymorphism profiles of the T3SS‐related genes had similar patterns. Additionally, naturally competent T3SS‐negative V. cholerae incorporated the ca. 47 kb gene cluster of T3SS, which had been integrated into a site on the chromosome by recombination. Therefore, it is suggested that horizontal gene transfer of T3SS‐related genes occurs among V. cholerae in natural ecosystems.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/1348-0421.12039</identifier><identifier>PMID: 23668604</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Bacteriology ; Cholera ; Cholera - microbiology ; Chromosomes, Bacterial ; Electrophoresis, Gel, Pulsed-Field ; Environmental Microbiology ; Gene Transfer, Horizontal ; Genes ; Genetic Variation ; Genomic Islands ; Genotype ; horizontal gene transfer ; Humans ; Membrane Transport Proteins - genetics ; Microbiology ; Molecular Typing ; Multigene Family ; natural transformation ; Polymorphism, Restriction Fragment Length ; Vibrio cholerae ; Vibrio cholerae - classification ; Vibrio cholerae - genetics ; Vibrio cholerae - isolation & purification ; Vibrio cholerae - pathogenicity ; Virulence Factors - genetics</subject><ispartof>Microbiology and immunology, 2013-05, Vol.57 (5), p.334-339</ispartof><rights>2013 The Societies and Wiley Publishing Asia Pty Ltd</rights><rights>2013 The Societies and Wiley Publishing Asia Pty Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5349-102282d1c2d1a792af9cb3162e9d8463346e62429f7c1e4d539a97bd4c68201f3</citedby><cites>FETCH-LOGICAL-c5349-102282d1c2d1a792af9cb3162e9d8463346e62429f7c1e4d539a97bd4c68201f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1348-0421.12039$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1348-0421.12039$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27922,27923,45572,45573,46407,46831</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23668604$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morita, Masatomo</creatorcontrib><creatorcontrib>Yamamoto, Shouji</creatorcontrib><creatorcontrib>Hiyoshi, Hirotaka</creatorcontrib><creatorcontrib>Kodama, Toshio</creatorcontrib><creatorcontrib>Okura, Masatoshi</creatorcontrib><creatorcontrib>Arakawa, Eiji</creatorcontrib><creatorcontrib>Alam, Munirul</creatorcontrib><creatorcontrib>Ohnishi, Makoto</creatorcontrib><creatorcontrib>Izumiya, Hidemasa</creatorcontrib><creatorcontrib>Watanabe, Haruo</creatorcontrib><title>Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae</title><title>Microbiology and immunology</title><addtitle>Microbiol Immunol</addtitle><description>ABSTRACT
Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS‐related genes were distributed among the various serogroups and pulsed‐field gel electrophoresis of NotI‐digested genomes showed genetic diversity in these strains. However, the restriction fragment length polymorphism profiles of the T3SS‐related genes had similar patterns. Additionally, naturally competent T3SS‐negative V. cholerae incorporated the ca. 47 kb gene cluster of T3SS, which had been integrated into a site on the chromosome by recombination. Therefore, it is suggested that horizontal gene transfer of T3SS‐related genes occurs among V. cholerae in natural ecosystems.</description><subject>Bacteriology</subject><subject>Cholera</subject><subject>Cholera - microbiology</subject><subject>Chromosomes, Bacterial</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Environmental Microbiology</subject><subject>Gene Transfer, Horizontal</subject><subject>Genes</subject><subject>Genetic Variation</subject><subject>Genomic Islands</subject><subject>Genotype</subject><subject>horizontal gene transfer</subject><subject>Humans</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Microbiology</subject><subject>Molecular Typing</subject><subject>Multigene Family</subject><subject>natural transformation</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae - classification</subject><subject>Vibrio cholerae - genetics</subject><subject>Vibrio cholerae - isolation & purification</subject><subject>Vibrio cholerae - pathogenicity</subject><subject>Virulence Factors - genetics</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9LHDEYhkOp1K313FsJ9NLLaH5NZnIsUnVAKwVbvYVM8o2NnU3WZIZ2-9c36-oeejEQAl-e94WPB6H3lBzRco4pF21FBKNHlBGuXqHFbvIaLQhv66qWhOyjtznfE8Ia1oo3aJ9xKVtJxAKtzmPyf2OYzIjvIACekgl5gITjgM3jaPIW-zya4DAEG50Pd-VnWq8Ad12HM9hUmBhwXucJltj5PCXfzxM47AP-4fvkI7Y_4wjJwDu0N5gxw-HTe4C-n365PjmvLq7OupPPF5WtuVAVJYy1zFFbrmkUM4OyPaeSgXKtkJwLCZIJpobGUhCu5sqopnfCypYROvAD9Gnbu0rxYYY86aXPFsayB8Q5ayq4YpwxSl9GeU0lJaJVBf34H3of5xTKIhuKcEGVIIU63lI2xZwTDHqV_NKktaZEb7zpjSW9saQfvZXEh6feuV-C2_HPogpQb4HffoT1S336srt8Lq62uSIF_uxyJv3SsuFNrW--nukbXn9r5O21PuX_AKjhrzk</recordid><startdate>201305</startdate><enddate>201305</enddate><creator>Morita, Masatomo</creator><creator>Yamamoto, Shouji</creator><creator>Hiyoshi, Hirotaka</creator><creator>Kodama, Toshio</creator><creator>Okura, Masatoshi</creator><creator>Arakawa, Eiji</creator><creator>Alam, Munirul</creator><creator>Ohnishi, Makoto</creator><creator>Izumiya, Hidemasa</creator><creator>Watanabe, Haruo</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201305</creationdate><title>Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae</title><author>Morita, Masatomo ; Yamamoto, Shouji ; Hiyoshi, Hirotaka ; Kodama, Toshio ; Okura, Masatoshi ; Arakawa, Eiji ; Alam, Munirul ; Ohnishi, Makoto ; Izumiya, Hidemasa ; Watanabe, Haruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5349-102282d1c2d1a792af9cb3162e9d8463346e62429f7c1e4d539a97bd4c68201f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Bacteriology</topic><topic>Cholera</topic><topic>Cholera - microbiology</topic><topic>Chromosomes, Bacterial</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Environmental Microbiology</topic><topic>Gene Transfer, Horizontal</topic><topic>Genes</topic><topic>Genetic Variation</topic><topic>Genomic Islands</topic><topic>Genotype</topic><topic>horizontal gene transfer</topic><topic>Humans</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Microbiology</topic><topic>Molecular Typing</topic><topic>Multigene Family</topic><topic>natural transformation</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae - classification</topic><topic>Vibrio cholerae - genetics</topic><topic>Vibrio cholerae - isolation & purification</topic><topic>Vibrio cholerae - pathogenicity</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morita, Masatomo</creatorcontrib><creatorcontrib>Yamamoto, Shouji</creatorcontrib><creatorcontrib>Hiyoshi, Hirotaka</creatorcontrib><creatorcontrib>Kodama, Toshio</creatorcontrib><creatorcontrib>Okura, Masatoshi</creatorcontrib><creatorcontrib>Arakawa, Eiji</creatorcontrib><creatorcontrib>Alam, Munirul</creatorcontrib><creatorcontrib>Ohnishi, Makoto</creatorcontrib><creatorcontrib>Izumiya, Hidemasa</creatorcontrib><creatorcontrib>Watanabe, Haruo</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morita, Masatomo</au><au>Yamamoto, Shouji</au><au>Hiyoshi, Hirotaka</au><au>Kodama, Toshio</au><au>Okura, Masatoshi</au><au>Arakawa, Eiji</au><au>Alam, Munirul</au><au>Ohnishi, Makoto</au><au>Izumiya, Hidemasa</au><au>Watanabe, Haruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiol Immunol</addtitle><date>2013-05</date><risdate>2013</risdate><volume>57</volume><issue>5</issue><spage>334</spage><epage>339</epage><pages>334-339</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><abstract>ABSTRACT
Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS‐related genes were distributed among the various serogroups and pulsed‐field gel electrophoresis of NotI‐digested genomes showed genetic diversity in these strains. However, the restriction fragment length polymorphism profiles of the T3SS‐related genes had similar patterns. Additionally, naturally competent T3SS‐negative V. cholerae incorporated the ca. 47 kb gene cluster of T3SS, which had been integrated into a site on the chromosome by recombination. Therefore, it is suggested that horizontal gene transfer of T3SS‐related genes occurs among V. cholerae in natural ecosystems.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>23668604</pmid><doi>10.1111/1348-0421.12039</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Microbiology and immunology, 2013-05, Vol.57 (5), p.334-339 |
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| source | MEDLINE; Wiley Online Library Free Content; Open Access Titles of Japan; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Bacteriology Cholera Cholera - microbiology Chromosomes, Bacterial Electrophoresis, Gel, Pulsed-Field Environmental Microbiology Gene Transfer, Horizontal Genes Genetic Variation Genomic Islands Genotype horizontal gene transfer Humans Membrane Transport Proteins - genetics Microbiology Molecular Typing Multigene Family natural transformation Polymorphism, Restriction Fragment Length Vibrio cholerae Vibrio cholerae - classification Vibrio cholerae - genetics Vibrio cholerae - isolation & purification Vibrio cholerae - pathogenicity Virulence Factors - genetics |
| title | Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae |
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